Shepherd's Purse Polyphenols Exert Its Anti-Inflammatory and Antioxidative Effects Associated with Suppressing MAPK and NF-κB Pathways and Heme Oxygenase-1 Activation.
Jinming PengTian-Yong HuJin LiJing DuKerui ZhuBaohui ChengKaikai LiPublished in: Oxidative medicine and cellular longevity (2019)
Shepherd's purse (Capsella bursa-pastoris (L.) Medik.), a wild herb as a traditional herbal medicine, has been proved with multiple healthy benefits. In this study, the chemical constituents of shepherd's purse were identified by UPLC-QTOF-MS/MS. The antioxidative and anti-inflammatory potential of shepherd's purse extract (SPE) were also investigated applying lipopolysaccharide- (LPS-) induced inflammation in RAW 264.7 macrophages and a carrageenan-induced mice paw edema model. Twenty-four chemical compounds were identified mainly including phenolic acids and flavonoids. The data also indicated SPE inhibited the productions of NO, PGE2, TNF-α, and IL-6 stimulated with LPS. In addition, SPE inhibited the increase of reactive oxygen species (ROS) and upregulated the expression of heme oxygenase-1 (HO-1). We further found that SPE inhibited the phosphorylation of P38 MAPK and activation of NF-κB. In vivo mice model also indicated that SPE showed strong antioxidative and anti-inflammatory activity.
Keyphrases
- ms ms
- anti inflammatory
- lps induced
- inflammatory response
- reactive oxygen species
- signaling pathway
- oxidative stress
- liquid chromatography tandem mass spectrometry
- pi k akt
- high fat diet induced
- diabetic rats
- poor prognosis
- toll like receptor
- dna damage
- high glucose
- type diabetes
- ultra high performance liquid chromatography
- cell death
- solid phase extraction
- endothelial cells
- nuclear factor
- drug induced
- risk assessment
- big data
- atomic force microscopy
- insulin resistance
- high resolution
- binding protein
- high speed