Preparation of the standard cell lines for reference mutations in cancer gene-panels by genome editing in HEK 293 T/17 cells.
Takayoshi SuzukiYoshinori TsukumoChie FurihataMikihiko NaitoArihiro KoharaPublished in: Genes and environment : the official journal of the Japanese Environmental Mutagen Society (2020)
These knock-in HEK293T/17 cells can be used as the reference mutant standards with a steady and continuous supply for NGS-based cancer panel tests from the JCRB cell bank. In addition, these cell lines can provide a tool for the functional analysis of targeted mutations in cancer-related genes in the isogenic background.