The Increased Apoptosis of Mesenchymal Stem Cells Mediated Osteopenia Due to Prenatal Nicotine Exposure in Female Offspring Rats via IGF1 Pathway.
Xufeng LiHao XiaoZhixin WuHui WangLiaobin ChenPublished in: Environmental toxicology (2024)
Nicotine exposure is a common adverse environment during pregnancy and causes developmental toxicity of long bones in offspring. However, the effect of prenatal nicotine exposure (PNE) on bone mass accumulation in female offspring and its mechanism remained to be further investigated. In this study, we constructed a PNE rat model and collected the long bone and the bone marrow mesenchymal stem cells (BMSCs) from female offspring rats for the detection of bone mass, cell apoptosis, and the expressions of osteogenesis- and apoptosis-related genes. The results revealed that PNE induced low bone mass in female offspring rats and was associated with the suppression of osteogenic function. Moreover, the apoptosis of BMSCs derived from the PNE female offspring rats was raised, and the expression ratio of apoptosis marker genes BAX/BCL-2 was significantly increased. Further, PNE inhibited the expression and function of insulin-like growth factor l (IGF1) signaling pathway in BMSCs. However, the exogenous IGF1 treatment partially ameliorated the increased apoptosis of BMSCs derived from the PNE female offspring rats. In conclusion, PNE induced low bone mass in female offspring rats, which was attributed to the increased apoptosis of BMSCs due to functional inhibition of IGF1 signaling pathway.
Keyphrases
- cell cycle arrest
- high fat diet
- pi k akt
- oxidative stress
- endoplasmic reticulum stress
- signaling pathway
- cell death
- bone mineral density
- mesenchymal stem cells
- induced apoptosis
- binding protein
- poor prognosis
- soft tissue
- bone regeneration
- diabetic rats
- smoking cessation
- pregnant women
- cell proliferation
- type diabetes
- epithelial mesenchymal transition
- bone loss
- adipose tissue
- high glucose
- skeletal muscle
- insulin resistance
- body composition
- long non coding rna
- genome wide
- single cell
- postmenopausal women
- metabolic syndrome
- wastewater treatment
- real time pcr
- drug induced