Cpf1-assisted efficient genomic integration of in vivo assembled DNA parts in Saccharomyces cerevisiae.

Zhen-Hai LiMin LiuFeng-Qing WangDong-Zhi Wei

Published in: Biotechnology letters (2018)

CRISPR-Cpf1 system allows for efficient genomic integration of in vivo assembled DNA parts in S. cerevisiae, and thus provides an alternative CRISPR-Cas method for metabolic pathway engineering in addition to CRISPR-Cas9 system previously reported for yeast.

Keyphrases

genome editing
crispr cas
saccharomyces cerevisiae
circulating tumor
cell free
single molecule
copy number
nucleic acid
gene expression
circulating tumor cells
dna methylation
genome wide