Development of a Novel High-Performance Thin Layer Chromatography-Based Method for the Simultaneous Quantification of Clinically Relevant Lipids from Cells and Tissue Extracts.
Michelle PinaultCyrille GuimaraesCéline Ben HassenJorge L Gutierrez-PajaresStéphan ChevalierCaroline GoupillePierre Bernard-SavaryPhilippe G FrankPublished in: Lipids (2020)
Lipids such as cholesterol, triacylglycerols, and fatty acids play important roles in the regulation of cellular metabolism and cellular signaling pathways and, as a consequence, in the development of various diseases. It is therefore important to understand how their metabolism is regulated to better define the components involved in the development of various human diseases. In the present work, we describe the development and validation of a high-performance thin layer chromatography (HPTLC) method allowing the separation and quantification of free cholesterol, cholesteryl esters, nonesterified fatty acids, and triacylglycerols. This method will be of interest as the quantification of these lipids in one single assay is difficult to perform.
Keyphrases
- fatty acid
- mass spectrometry
- induced apoptosis
- liquid chromatography
- signaling pathway
- endothelial cells
- tandem mass spectrometry
- transcription factor
- high throughput
- low density lipoprotein
- oxidative stress
- epithelial mesenchymal transition
- ms ms
- high resolution
- induced pluripotent stem cells
- simultaneous determination