Ginkgetin effectively mitigates collagen and AA-induced platelet activation via PLCγ2 but not cyclic nucleotide-dependent pathway in human.
Chih-Wei HsiaLan-Hsin ShuAi-Wei LeeOanh-Thi TranChih-Hao YangTing-Lin YenWei-Chieh HuangChih-Hsuan HsiaThanasekaran JayakumarKuan-Rau ChiouJoen-Rong SheuPublished in: Journal of cellular and molecular medicine (2024)
Platelets assume a pivotal role in the cardiovascular diseases (CVDs). Thus, targeting platelet activation is imperative for mitigating CVDs. Ginkgetin (GK), from Ginkgo biloba L, renowned for its anticancer and neuroprotective properties, remains unexplored concerning its impact on platelet activation, particularly in humans. In this investigation, we delved into the intricate mechanisms through which GK influences human platelets. At low concentrations (0.5-1 μM), GK exhibited robust inhibition of collagen and arachidonic acid (AA)-induced platelet aggregation. Intriguingly, thrombin and U46619 remained impervious to GK's influence. GK's modulatory effect extended to ATP release, P-selectin expression, intracellular calcium ([Ca 2+ ]i) levels and thromboxane A 2 formation. It significantly curtailed the activation of various signaling cascades, encompassing phospholipase Cγ2 (PLCγ2)/protein kinase C (PKC), phosphoinositide 3-kinase/Akt/glycogen synthase kinase-3β and mitogen-activated protein kinases. GK's antiplatelet effect was not reversed by SQ22536 (an adenylate cyclase inhibitor) or ODQ (a guanylate cyclase inhibitor), and GK had no effect on the phosphorylation of vasodilator-stimulated phosphoprotein Ser157 or Ser239 . Moreover, neither cyclic AMP nor cyclic GMP levels were significantly increased after GK treatment. In mouse studies, GK notably extended occlusion time in mesenteric vessels, while sparing bleeding time. In conclusion, GK's profound impact on platelet activation, achieved through inhibiting PLCγ2-PKC cascade, culminates in the suppression of downstream signaling and, ultimately, the inhibition of platelet aggregation. These findings underscore the promising therapeutic potential of GK in the CVDs.
Keyphrases
- protein kinase
- cardiovascular disease
- endothelial cells
- signaling pathway
- poor prognosis
- atrial fibrillation
- cell proliferation
- escherichia coli
- metabolic syndrome
- blood brain barrier
- autism spectrum disorder
- wound healing
- pseudomonas aeruginosa
- radiation therapy
- coronary artery disease
- drug induced
- long non coding rna
- binding protein
- reactive oxygen species