Genomic surveillance of SARS-CoV-2 Spike gene by sanger sequencing.
Tiago Souza SallesAndrea Cony CavalcantiFábio Burack da CostaVanessa Zaquieu DiasLeandro Magalhães de SouzaMarcelo Damião Ferreira de MenesesJosé Antônio Suzano da SilvaCinthya Domingues AmaralJhonatan Ramos FelixDuleide Alves PereiraStefanella BoattoMaria Angélica Arpon Marandino GuimarãesDavis Fernandes FerreiraRenata Campos AzevedoPublished in: PloS one (2022)
The SARS-CoV-2 responsible for the ongoing COVID pandemic reveals particular evolutionary dynamics and an extensive polymorphism, mainly in Spike gene. Monitoring the S gene mutations is crucial for successful controlling measures and detecting variants that can evade vaccine immunity. Even after the costs reduction resulting from the pandemic, the new generation sequencing methodologies remain unavailable to a large number of scientific groups. Therefore, to support the urgent surveillance of SARS-CoV-2 S gene, this work describes a new feasible protocol for complete nucleotide sequencing of the S gene using the Sanger technique. Such a methodology could be easily adopted by any laboratory with experience in sequencing, adding to effective surveillance of SARS-CoV-2 spreading and evolution.