The identification of T and B lymphocytes has relied on using antibodies against different biomarkers as the gold standard. Emerging small molecule-based fluorescent probes have the potential to replace antibodies. Herein, we report the first human-T-cell-selective fluorescent probe, Mito thermo yellow (MTY), achieving the live T cells' distinction from B cells, which was previously impossible without the help of antibodies. The unexpected cell selectivity of MTY is attributed to the higher mitochondria mass and membrane potential of T cells over B cells. This study enriches the toolbox for live cell distinction from complex cell communities.
Keyphrases
- fluorescent probe
- living cells
- small molecule
- endothelial cells
- single cell
- single molecule
- induced pluripotent stem cells
- pluripotent stem cells
- stem cells
- protein protein
- risk assessment
- mesenchymal stem cells
- fluorescence imaging
- quantum dots
- bone marrow
- human health
- silver nanoparticles
- structural basis
- nucleic acid