Login / Signup

Near-Quantitative Preparation of Short Single-Stranded DNA Circles.

Victoria E PaluzziCuizheng ZhangChengde Mao
Published in: Angewandte Chemie (International ed. in English) (2023)
Small, single-stranded DNA (ssDNA) circles have many applications, such as templating rolling circle amplification (RCA), capturing microRNAs, and scaffolding DNA nanostructures. However, it is challenging to prepare such ssDNA circles, particularly when the DNA size becomes very small (e.g. a 20 nucleotide (nt) long ssDNA circle). Often, such short ssDNA dominantly form concatemers (either linear or circular) due to intermolecular ligation, instead of forming monomeric ssDNA circles by intramolecular ligation. Herein, a simple method to overcome this problem by designing the complementary linker molecules is reported. It is demonstrated that ssDNA, as short as 16 nts, can be enzymatically ligated (by the commonly used T4 DNA ligase) into monomeric ssDNA circles at high concentration (100 μM) with high yield (97 %). This method does not require any special sequence, thus, it is expected to be generally applicable. The experimental protocol is identical to regular DNA ligation, thus, is expected to be user friendly for general chemists and biologists.
Keyphrases
  • circulating tumor
  • cell free
  • nucleic acid
  • single molecule
  • randomized controlled trial
  • circulating tumor cells
  • mass spectrometry
  • amino acid
  • energy transfer