Toxicity evaluation of choline ionic liquid-based nanocarriers of pharmaceutical agents for lung treatment.
Katarzyna NiesytoMagdalena SkoniecznaMałgorzata Adamiec-OrganiściokDorota NeugebauerPublished in: Journal of biomedical materials research. Part B, Applied biomaterials (2023)
In vitro cytotoxicity evaluation of linear copolymer (LC) containing choline ionic liquid units and its conjugates with an antibacterial drug in anionic form, that is, p-aminosalicylate (LC_PAS), clavulanate (LC_CLV), or piperacillin (LC_PIP) was carried out. These systems were tested against normal: human bronchial epithelial cells (BEAS-2B), and cancers: adenocarcinoma human alveolar basal epithelial cells (A549), and human non-small cell lung carcinoma cell line (H1299). Cells viability, after linear copolymer LC and their conjugates addition for 72 h, was measured at concentration range of 3.125-100 μg/mL. The MTT test allowed the designation of IC 50 index, which was higher for BEAS-2B, and significantly lower in the case of cancer cell lines. The cytometric analyzes, that is, Annexin-V FITC apoptosis assay and cell cycle analysis as well as gene expression measurements for interleukins IL6 and IL8 were carried out, and showed pro-inflammatory activity of tested compounds toward cancer cells, while it was not observed against normal cell line.
Keyphrases
- ionic liquid
- endothelial cells
- cell cycle
- gene expression
- simultaneous determination
- induced pluripotent stem cells
- pluripotent stem cells
- room temperature
- oxidative stress
- mass spectrometry
- cell cycle arrest
- liquid chromatography
- cell proliferation
- drug delivery
- squamous cell carcinoma
- induced apoptosis
- emergency department
- stem cells
- dna methylation
- endoplasmic reticulum stress
- locally advanced
- tandem mass spectrometry
- squamous cell
- replacement therapy