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Laboratory coagulation tests and recombinant porcine factor VIII: A United Kingdom Haemophilia Centre Doctors' Organisation guideline.

Annette Elizabeth BowyerElaine GrayAnna LowePaul MurphySean PlattonAnne RiddellPratima ChowdaryWilliam A LesterPeter Vincent Jenkins
Published in: Haemophilia : the official journal of the World Federation of Hemophilia (2022)
A validated one-stage clotting FVIII assay is recommended for the measurement and regular monitoring of rpFVIII. Assessment of cross-reacting rpFVIII inhibitors by one-stage porcine Bethesda assay should be performed as part of the initial diagnosis of AHA or prior to treatment with rpFVIII. Available data show that chromogenic FVIII assays underestimate rpFVIII and this should be considered if measurement of rpFVIII is required in patients receiving Emicizumab.
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