Login / Signup

Holotype sequencing of Silvataresholzenthali Rázuri-Gonzales, Ngera & Pauls, 2022 (Trichoptera, Pisuliidae).

Jacqueline HeckenhauerErnesto Rázuri-GonzalesFrancois Ngera MwangiJulio SchneiderSteffen U Pauls
Published in: ZooKeys (2023)
While DNA barcodes are increasingly provided in descriptions of new species, the whole mitochondrial and nuclear genomes are still rarely included. This is unfortunate because whole genome sequencing of holotypes allows perpetual genetic characterization of the most representative specimen for a given species. Thus, de novo genomes are invaluable additional diagnostic characters in species descriptions, provided the structural integrity of the holotype specimens remains intact. Here, we used a minimally invasive method to extract DNA of the type specimen of the recently described caddisfly species Silvataresholzenthali Rázuri-Gonzales, Ngera & Pauls, 2022 (Trichoptera: Pisuliidae) from the Democratic Republic of the Congo. A low-cost next generation sequencing strategy was used to generate the complete mitochondrial and draft nuclear genome of the holotype. The data in its current form is an important extension to the morphological species description and valuable for phylogenomic studies.
Keyphrases
  • low cost
  • oxidative stress
  • minimally invasive
  • circulating tumor
  • genetic diversity
  • single molecule
  • cross sectional
  • copy number
  • big data
  • dna methylation
  • deep learning
  • anti inflammatory
  • nucleic acid
  • case control