Enhanced Antitumoral Activity and Photoacoustic Imaging Properties of AuNP-Enriched Endothelial Colony Forming Cells on Melanoma.
Paolo ArmanettiAnastasia ChillàFrancesca MargheriAlessio BiagioniLuca MenichettiGiancarlo MargheriFulvio RattoSonia CentiFrancesca BianchiniMirko SeveriRita TraversiDaniele BaniMatteo LulliTommaso Del RossoAlessandra MocaliElisabetta RovidaMario Del RossoGabriella FibbiAnna LaurenzanaPublished in: Advanced science (Weinheim, Baden-Wurttemberg, Germany) (2020)
Near infrared (NIR)-resonant gold nanoparticles (AuNPs) hold great promise in cancer diagnostics and treatment. However, translating the theranostic potential of AuNPs into clinical applications still remains a challenge due to the difficulty to improve the efficiency and specificity of tumor delivery in vivo as well as the clearance from liver and spleen to avoid off target toxicity. In this study, endothelial colony forming cells (ECFCs) are exploited as vehicles to deliver AuNPs to tumors. It is first demonstrated that ECFCs display a great capability to intake AuNPs without losing viability, and exert antitumor activity per se. Using a human melanoma xenograft mouse model, it is next demonstrated that AuNP-loaded ECFCs retain their capacity to migrate to tumor sites in vivo 1 day after injection and stay in the tumor mass for more than 1 week. In addition, it is demonstrated that ECFC-loaded AuNPs are efficiently cleared by the liver over time and do not elicit any sign of damage to healthy tissue.
Keyphrases
- induced apoptosis
- endothelial cells
- gold nanoparticles
- mouse model
- cell cycle arrest
- drug delivery
- fluorescence imaging
- oxidative stress
- photodynamic therapy
- high resolution
- signaling pathway
- endoplasmic reticulum stress
- clinical trial
- papillary thyroid
- cell death
- machine learning
- climate change
- big data
- pluripotent stem cells
- deep learning
- lymph node metastasis
- induced pluripotent stem cells
- childhood cancer
- energy transfer
- squamous cell
- basal cell carcinoma