Spherical nucleic acid reporter-based cascade CRISPR/Cas12a amplifier for stable and sensitive biosensing of circulating tumor DNA.
Min ZhouYao YinYuyan ShiZhaoxin HuangYu ShiMei ChenGuoliang KeXiao-Bing ZhangPublished in: Chemical communications (Cambridge, England) (2022)
Stable and sensitive ctDNA biosensing in complex biological fluid is highly important but still remains a challenge. Herein, we develop a spherical nucleic acid reporter-based cascade CRISPR/Cas12a amplifier with improved stability and sensitivity (5 orders of magnitude).