Modulation of the microhomology-mediated end joining pathway suppresses large deletions and enhances homology-directed repair following CRISPR-Cas9-induced DNA breaks.
Baolei YuanChongwei BiYeteng TianJincheng WangYiqing JinKhaled AlsayeghMuhammad TehseenGang YiXuan ZhouYanjiao ShaoFernanda Vargas RomeroWolfgang FischleJuan Carlos Izpisua BelmonteSamir HamdanYanyi HuangMo LiPublished in: BMC biology (2024)
Our findings reveal the contrasting roles of RPA and POLQ in Cas9-induced LD and HDR, suggesting new strategies for safer and more precise genome editing.