This study examined the effects of an 8-week horizontal speed deceleration training (HSDT) programme in combination with regular handball-specific training as compared with handball-specific training only in measures of physical fitness in male youth handball players. Thirty-nine players were randomly assigned to either an HSDT group ( n = 18; 15.55 ± 0.24 years) or an active-control group (CG; n = 21; 14.59 ± 0.23 years). The results showed significant and large between-group differences at post-test in countermovement jump, change-of-direction speed, and repeated sprint ability (RSA) (all p < 0.01; d = 2.04 and 1.37, 1.39, 1.53, and 1.53 for the CMJ, 505 CoD, RSA best , RSA average , and RSA total performances, respectively). The post-hoc-analysis demonstrated significant and large improvements in all measures of physical fitness in the HSDT group (∆2.49% to 16,25%; d = 1.01 to 1,70; all p < 0,01). The CG, however, failed to reach any significant difference in all measures of physical fitness ((∆0.31% to 1.98%; d = 0.15 to 0.22; p = 0.379; p > 0.05). To summarise, an 8-week in-season HSDT programme alongside regular handball-specific training yielded positive effects on various performance measures including jumping ability, CoD speed, and RSA, when compared to handball-specific training alone. These results highlight the potential benefits of integrating HSDT into the training regimen of youth handball athletes during the competitive season.