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Metabologenomics-Inspired Discovery and Combinatorial Biosynthesis-Based Diversification of Fungal O -Glycosylated Depsides.

Hu YangZhuo ShangYingying ChenFeng LiKunlong LiHongjie ZhuMing PengJiafan YangCunlei CaiJianhua Ju
Published in: Organic letters (2024)
Through metabologenomics mining, we prioritized Exophiala xenobiotica SDU 039, a deep-sea sediment-derived fungus producing O -glycosylated depsides ( 1 - 9 ), including seven new species with varying aliphatic chains. Heterologous expression validated the exo gene cluster, and in vitro enzyme assays elucidated the function of glycosyltransferase ExoC. The chemical diversity of O -glycosylated depsides is expanded by combinatorial biosynthesis using homologues depside biosynthetic genes and in vitro transformation with ExoC and different sugars as substrate.
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