miR-148a regulation interferes in inflammatory cytokine and parasitic load in canine leishmaniasis.
Gabriela Torres RebechJaqueline Poleto BragatoSidnei Ferro CostaJéssica Henrique de FreitasMarilene Oliveira Dos SantosMatheus Fujimura SoaresFlávia de Rezende EugênioPaulo Sérgio Patto Dos SantosValéria Marçal Felix de LimaPublished in: PLoS neglected tropical diseases (2023)
Canine leishmaniasis (CanL) is a severe public health threat. Infected animals mediate transmission of the Leishmania protozoan to humans via the sandfly's bite during a blood meal. CanL progression depends on the degree of suppression of the immune response, possibly associated with microRNAs (miR), which can modulate mRNA translation into proteins and (consequently) regulate cell function. Increased miR-148a in splenic leukocytes (SL) of dogs with CanL was observed in previous studies, and in silico analysis, identified possible pathways involved in immune response regulation that are affected by this miR. Therefore, we evaluated the involvement of miR-148a in the regulation of TNF-α, IL-6, IL-12, IL-1β, iNOS, MHCII, CD80, CD3, T-bet, and GATA-3 transcription factors and their relationship with parasite load in SL of dogs with CanL. Splenic leukocytes obtained from healthy and diseased dogs were transfected with miR-148a mimic and inhibitor oligonucleotides. After 48 hours, expression levels of MHCII, CD80, iNOS, CD3, T-bet, and GATA-3 were evaluated by flow cytometry, and concentrations of TNF-α, IL-12, IL-6, and IL-1β were measured in culture supernatants by capture enzyme-linked immunosorbent assays. Transfection of SL with miR-148a mimics decreased iNOS levels in cells and TNF-α, IL-6, and IL-12 in the supernatants of cultured SL from CanL dogs. Interestingly, transfection with miR-148a inhibitor decreased parasite load in SL cells. These results suggest a direct or not regulatory role of this miR in the immune response to Leishmania infantum infection. We conclude that miR-148a can modulate immune responses by regulating inflammatory cytokines during CanL. Our results contribute to understanding the complex host/parasite interaction in CanL and could assist the development of treatments.
Keyphrases
- cell proliferation
- long non coding rna
- immune response
- long noncoding rna
- transcription factor
- poor prognosis
- public health
- rheumatoid arthritis
- induced apoptosis
- oxidative stress
- flow cytometry
- cell death
- toll like receptor
- nitric oxide
- binding protein
- early onset
- nitric oxide synthase
- molecular dynamics simulations
- molecular docking
- life cycle
- nucleic acid