Rapid identification of mycobacteria from positive MGIT broths of primary cultures by MALDI-TOF mass spectrometry.
Tsi-Shu HuangChia-Chien LeeHui-Zin TuSusan Shin-Jung LeePublished in: PloS one (2018)
Based on a simplified protocol, we showed that MALDI-TOF MS can be used for rapid identification of NTM directly from primary MGIT cultures within the routine clinical laboratory workflow. However, we recommend an initial ICA test to screen for M.tuberculosis complex, due to a low identification rate of M. tuberculosis in the presence of polymicrobial cultures using MALDI-TOF.
Keyphrases
- mass spectrometry
- liquid chromatography
- gas chromatography
- high performance liquid chromatography
- capillary electrophoresis
- high resolution
- mycobacterium tuberculosis
- bioinformatics analysis
- randomized controlled trial
- emergency department
- loop mediated isothermal amplification
- clinical practice
- electronic health record
- tandem mass spectrometry
- simultaneous determination
- drug induced
- hiv infected