A multiplex-NGS approach to identifying respiratory RNA viruses during the COVID-19 pandemic.
Natalia RamosYanina PanzeraSandra FrabasileGonzalo TomásLucía CallerosAna MarandinoNatalia GoñiClaudia TecheraSofía GreccoEddie FuquesLeticia CoppolaViviana RamasMaria Noelia MorelCristina MogdasyHéctor ChiparelliJuan ArbizaRuben PérezAdriana DelfraroPublished in: Archives of virology (2023)
A methodological approach based on reverse transcription (RT)-multiplex PCR followed by next-generation sequencing (NGS) was implemented to identify multiple respiratory RNA viruses simultaneously. A convenience sampling from respiratory surveillance and SARS-CoV-2 diagnosis in 2020 and 2021 in Montevideo, Uruguay, was analyzed. The results revealed the cocirculation of SARS-CoV-2 with human rhinovirus (hRV) A, B and C, human respiratory syncytial virus (hRSV) B, influenza A virus, and metapneumovirus B1. SARS-CoV-2 coinfections with hRV or hRSV B and influenza A virus coinfections with hRV C were identified in adults and/or children. This methodology combines the benefits of multiplex genomic amplification with the sensitivity and information provided by NGS. An advantage is that additional viral targets can be incorporated, making it a helpful tool to investigate the cocirculation and coinfections of respiratory viruses in pandemic and post-pandemic contexts.
Keyphrases
- sars cov
- respiratory syncytial virus
- respiratory syndrome coronavirus
- endothelial cells
- real time pcr
- high throughput
- respiratory tract
- induced pluripotent stem cells
- nucleic acid
- public health
- copy number
- pluripotent stem cells
- coronavirus disease
- transcription factor
- healthcare
- single cell
- dna methylation
- social media