Polymer-Based Terbium Complex as a Fluorescent Probe for Cancer Antigen 125 Detection: A Promising Tool for Early Diagnosis of Ovarian Cancer.
Magda M MohamedHisham GamalAkram El-DidamonyAhmed O YoussefEsraa ElshahatEkram H MohamedMohamed S AttiaPublished in: ACS omega (2024)
A novel photoprobe, Tb-acetylacetone (Tb-ACAC) doped within a modified epoxy cellulose polymer immobilized with CA-125 monoclonal antibody, offers an accurate and highly selective method for early ovarian cancer (OC) diagnosis by detecting cancer antigen 125 (CA-125) in serum samples. This approach leverages quenching of the Tb-ACAC luminescence upon binding to CA-125. Characterization of the photoprobe film through UV-vis and fluorescence measurements confirmed the presence of Tb-ACAC within the polymer matrix. In aqueous solution (pH 6.8, λ ex = 365 nm), the characteristic emission band of Tb-ACAC at λ em = 546.2 nm exhibited significant quenching upon CA-125 binding. This quenching effect enabled the sensitive and specific detection of CA-125 in diverse serum samples from OC patients, demonstrating the applicability, simplicity, and effectiveness of this novel approach.
Keyphrases
- mycobacterium tuberculosis
- aqueous solution
- energy transfer
- fluorescent probe
- monoclonal antibody
- papillary thyroid
- protein kinase
- quantum dots
- end stage renal disease
- photodynamic therapy
- randomized controlled trial
- living cells
- chronic kidney disease
- systematic review
- newly diagnosed
- ejection fraction
- squamous cell
- high resolution
- squamous cell carcinoma
- ionic liquid
- prognostic factors
- young adults
- loop mediated isothermal amplification
- lymph node metastasis
- gold nanoparticles
- light emitting
- transcription factor
- binding protein
- visible light