Metabolic exploration of the developmental abnormalities and neurotoxicity of Esculentoside B, the main toxic factor in Phytolaccae radix.
Sipei RenZhichao ZhangQinyang SongZhaoyang RenJian XiaoLuqi LiQiang ZhangPublished in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (2023)
P: radix is a perennial herb, and its extracts have various biological properties that make it a potential candidate for the treatment of tumors, edema, and lymphatic stasis. However, the main factor contributing to its toxicity are not clear. Here, we used a zebrafish toxicological model to study the main toxicity factor of P. radix and explore the potential mechanisms involved. The results revealed that Esculentoside B was the major toxic factor of P. radix. Exposure of zebrafish larvae to Esculentoside B caused developmental abnormalities, neurotoxicity and altered locomotor behavior. The combination of AChE activity and the expression levels of genes relevant to CNS development demonstrated that Esculentoside B is neurotoxic to zebrafish larvae, impairs their CNS development, and that AChE may be a toxic target of Esculentoside B. Metabolomic analysis has revealed that Esculentoside B exposure can disrupt D-Amino acid metabolism, protein export, autophagy, and mTOR signaling pathways in zebrafish larvae. These findings provide insights into the molecular mechanisms underlying EsB-induced neurotoxicity in zebrafish, which can facilitate further research and development of P. radix for safe consumption.
Keyphrases
- amino acid
- oxidative stress
- signaling pathway
- aedes aegypti
- single cell
- blood brain barrier
- poor prognosis
- lymph node
- cell death
- drosophila melanogaster
- endoplasmic reticulum stress
- human health
- climate change
- diabetic rats
- epithelial mesenchymal transition
- transcription factor
- long non coding rna
- risk assessment
- smoking cessation
- oxide nanoparticles