Comprehensive lipidome of human plasma using minimal sample manipulation by liquid chromatography coupled with mass spectrometry.
Bebiana C SousaZulema Gonzalez KleinDiane TaylorGreg WestAveline Neo HuipengMichael J O WakelamAndrea F Lopez-ClavijoPublished in: Rapid communications in mass spectrometry : RCM (2023)
The comprehensive analysis of the lipidome addressed here of glycerolipids, GPs, sterols and SPs is in good agreement with previously reported results, in the NIST SRM 1950 sample, by other laboratories. Ten lipid subclasses LPS, LPI, alkyl-lysophosphatidic acid/alkenyl-lysophosphatidic acid, alkyl-lysophosphatidylethanolamine/alkenyl-lysophosphatidylethanolamine, dhCer (d18:0), SPB (d18:1), dhSPB (d18:0) and SPBP (d18:2) have been detected using this comprehensive method and are uniquely reported here.
Keyphrases
- liquid chromatography
- mass spectrometry
- ionic liquid
- high resolution mass spectrometry
- tandem mass spectrometry
- inflammatory response
- high resolution
- high performance liquid chromatography
- gas chromatography
- simultaneous determination
- capillary electrophoresis
- fatty acid
- anti inflammatory
- solid phase extraction
- visible light