Study of Osteoarthritis-Related Hub Genes Based on Bioinformatics Analysis.
Zhengqing ZhuLei ZhongRonghang LiYuzhe LiuXiangrun ChenZhaoyan LiLanfeng HuangPublished in: BioMed research international (2020)
Osteoarthritis (OA) is a common cause of morbidity and disability worldwide. However, the pathogenesis of OA is unclear. Therefore, this study was conducted to characterize the pathogenesis and implicated genes of OA. The gene expression profiles of GSE82107 and GSE55235 were downloaded from the Gene Expression Omnibus database. Altogether, 173 differentially expressed genes including 68 upregulated genes and 105 downregulated genes in patients with OA were selected based on the criteria of ∣log fold-change | >1 and an adjusted p value < 0.05. Protein-protein interaction network analysis showed that FN1, COL1A1, IGF1, SPP1, TIMP1, BGN, COL5A1, MMP13, CLU, and SDC1 are the top ten genes most closely related to OA. Quantitative reverse transcription-polymerase chain reaction showed that the expression levels of COL1A1, COL5A1, TIMP1, MMP13, and SDC1 were significantly increased in OA. This study provides clues for the molecular mechanism and specific biomarkers of OA.
Keyphrases
- bioinformatics analysis
- knee osteoarthritis
- genome wide
- gene expression
- genome wide identification
- network analysis
- dna methylation
- small molecule
- rheumatoid arthritis
- protein protein
- transcription factor
- genome wide analysis
- emergency department
- poor prognosis
- cell proliferation
- mass spectrometry
- long non coding rna
- copy number
- signaling pathway
- binding protein