Green-Emitting Rhodamine Dyes for Vital Labeling of Cell Organelles Using STED Super-Resolution Microscopy.
Florian GrimmShamil NizamovVladimir N BelovPublished in: Chembiochem : a European journal of chemical biology (2019)
Fluorescence microscopy reveals the localization, spatial distribution, and temporal dynamics of the specifically labeled organelles in living cells. Labeling with exogenous conjugates prepared from fluorescent dyes and small molecules (ligands) is an attractive alternative to the use of fluorescent proteins, but proved to be challenging due to insufficient cell-permeability of the probes, unspecific staining, or low dye brightness. We evaluated four green-emitting rhodamine dyes and their conjugates intended for the specific labeling of lysosomes, mitochondria, tubulin, and actin in living cells. The imaging performance of the probes in living human fibroblasts has been studied by using confocal and stimulated emission depletion (STED) super-resolution microscopy with a commercial 595 nm STED laser. Two bright and photostable dyes (LIVE 510 and LIVE 515) provide specific and versatile staining.
Keyphrases
- living cells
- fluorescent probe
- single molecule
- high resolution
- high speed
- optical coherence tomography
- aqueous solution
- endothelial cells
- single cell
- high throughput
- cell therapy
- label free
- quantum dots
- cancer therapy
- cell death
- photodynamic therapy
- drug delivery
- stem cells
- pluripotent stem cells
- reactive oxygen species
- extracellular matrix
- endoplasmic reticulum
- fluorescence imaging