Periostin-modified bone marrow mesenchymal stem cells from osteoporotic rats promote alveolar bone regeneration.
Doudou LiWeiwei ZhouMeng CaoPublished in: Journal of molecular histology (2019)
Bone regeneration is impaired in patients with osteoporosis. Previous studies have shown that periostin (Postn) shows great potential in bone regeneration treatments. However, the role of Postn in bone marrow mesenchymal stem cells (BMMSCs) remains to be elucidated. In this study, we isolated BMMSCs from ovariectomized rats (OVX-BMMSCs) and normal rats. Then, the expression levels of Postn and osteogenesis in OVX-BMMSCs were detected by alizarin red and alkaline phosphatase substrate staining, qPCR, and western blotting. We found that the levels of Postn in OVX-BMMSCs were significantly reduced. Furthermore, Postn overexpression in OVX-BMMSCs using recombinant lentivirus could improve the expression of alkaline phosphatase, runt-related transcription factor 2, and osteocalcin and reduce the expression of sclerostin. Besides, micro-computed tomography analysis, hematoxylin-eosin, and Masson's staining showed that the healing of the alveolar bone defect in osteoporotic rats could be promoted using Postn-modified OVX-BMMSC sheets. In conclusion, Postn-modified OVX-BMMSCs might restore the osteogenic capacity and promote alveolar bone regeneration, which may serve as a new therapeutic approach for bone regeneration in osteoporosis.
Keyphrases
- bone regeneration
- bone marrow
- transcription factor
- poor prognosis
- bone mineral density
- computed tomography
- mesenchymal stem cells
- postmenopausal women
- stem cells
- magnetic resonance imaging
- south africa
- long non coding rna
- risk assessment
- climate change
- magnetic resonance
- flow cytometry
- human health
- drug induced
- image quality
- case control