Definition and Characterization of SOX11-Derived T Cell Epitopes towards Immunotherapy of Glioma.
Yibin LiuAnna KeibBrigitte NeuberLei WangAngelika Beate RiemerMaria BonsackAngela Hückelhoven-KraussAnita SchmittCarsten Müller-TidowMichael SchmittPublished in: International journal of molecular sciences (2023)
The transcription factor SOX11 is a tumor-associated antigen with low expression in normal cells, but overexpression in glioblastoma (GBM). So far, conventional surgery, chemotherapy, and radiotherapy have not substantially improved the dismal prognosis of relapsed/refractory GBM patients. Immunotherapy is considered a promising strategy against GBM, but there is a fervent need for better immunotargets in GBM. To this end, we performed an in silico prediction study on SOX11, which primarily yielded ten promising HLA-A*0201-restricted peptides derived from SOX11. We defined a novel peptide FMACSPVAL, which had the highest score according to in silico prediction (6.02 nM by NetMHC-4.0) and showed an exquisite binding affinity to the HLA-A*0201 molecule in the peptide-binding assays. In the IFN-γ ELISPOT assays, FMACSPVAL demonstrated a high efficiency for generating SOX11-specific CD8 + T cells. Nine out of thirty-two healthy donors showed a positive response to SOX11, as assessed by the ELISPOT assays. Therefore, this novel antigen peptide epitope seems to be promising as a target for T cell-based immunotherapy in GBM. The adoptive transfer of in vitro elicited SOX11-specific CD8 + T cells constitutes a potential approach for the treatment of GBM patients.
Keyphrases
- transcription factor
- stem cells
- end stage renal disease
- dna binding
- newly diagnosed
- ejection fraction
- chronic kidney disease
- high efficiency
- peritoneal dialysis
- prognostic factors
- high throughput
- squamous cell carcinoma
- molecular docking
- binding protein
- cell proliferation
- minimally invasive
- poor prognosis
- locally advanced
- early stage
- immune response
- dendritic cells
- coronary artery disease
- oxidative stress
- induced apoptosis
- mass spectrometry
- acute coronary syndrome
- signaling pathway
- single cell
- photodynamic therapy
- climate change
- cell death
- radiation induced
- genome wide identification
- cell cycle arrest
- hodgkin lymphoma
- percutaneous coronary intervention
- patient reported
- human health