Tangshen Formula Attenuates Colonic Structure Remodeling in Type 2 Diabetic Rats.
Pengmin ChenJingbo ZhaoHaojun ZhangXin YangTingting ZhaoHuicun ZhangMeihua YanLin PanXin LiYun ZhangPing LiPublished in: Evidence-based complementary and alternative medicine : eCAM (2017)
Aim. This study investigated the effect and mechanism of the Chinese herbal medicine Tangshen Formula (TSF) on GI structure remodeling in the rat model of diabetes. Methods. Type 2 diabetic rats were used. Wet weight per unit length, layer thicknesses, levels of collagens I and III, nuclear factor kappa B (NF-κB), interferon-γ (IFN-γ), interleukin-6 (IL-6), transforming growth factor-β1 (TGF-β1), and Smad2/3 expression in the rat colon were measured. Results. Compared with the control group animals, wet weight and layer thicknesses of the colon increased, and expressions of collagens I and III, NF-κB, IFN-γ, IL-6, TGF-β1, and Smad2/3 increased significantly in the diabetic animals. TSF inhibited increase in colonic wet weight and layer thicknesses, downregulated expressions of collagens I and III in the mucosal layer, and downregulated expressions of NF-κB, IFN-γ, IL-6, TGF-β1, and Smad2/3 in the colon wall. Furthermore, level of expression of NF-κB was associated with those of TGF-β1 and Smad2/3. Expression of TGF-β1 was associated with the most histomorphometric parameters including colonic weight, mucosal and muscle thicknesses, and levels of collagens I and III in mucosal layer. Conclusion. TSF appears to attenuate colonic structure remodeling in type 2 diabetic rats through inhibiting the overactivated pathway of NF-κB, thus reducing expressions of TGF-β1.
Keyphrases
- transforming growth factor
- diabetic rats
- nuclear factor
- oxidative stress
- epithelial mesenchymal transition
- signaling pathway
- ulcerative colitis
- toll like receptor
- poor prognosis
- lps induced
- weight loss
- body mass index
- dendritic cells
- physical activity
- pi k akt
- type diabetes
- immune response
- weight gain
- body weight
- cardiovascular disease
- skeletal muscle
- binding protein
- human milk
- high resolution
- mass spectrometry
- cell proliferation
- atomic force microscopy
- low birth weight