Marinobacter sediminicola sp. nov. and Marinobacter xiaoshiensis sp. nov., Isolated from Coastal Sediment.

Two Gram-stain-negative, facultative anaerobic, rod-shaped, motile bacterial strains, designated F26243 T and F60267 T were isolated from coastal sediment in Weihai, China. Strains F26243 T and F60267 T were grown at 4-40 °C (optimum 33 °C), pH 7.0-9.5 and pH 6.5-9.5 (optimum at pH 7.0), in the presence of 1.0-7.0% (w/v) NaCl (optimum 2.5%) and 1.0-12.0% (w/v) NaCl (optimum 2.0%), respectively. The 16S rRNA gene sequences phylogenetic analysis showed that strains F26243 T and F60267 T are closely related to the genus Marinobacter and exhibited the highest sequence similarities to Marinobacter salexigens HJR7 T (97.7% and 98.0%, respectively), the similarity between two isolates was 96.7%. Strains F26243 T and F60267 T displayed genomic DNA G + C content of 53.6% and 53.8%, respectively. When compared to the M. salexigens HJR7 T , the average nucleotide identity (ANI) values were 83.7% and 84.1%, and the percentage of conserved proteins (POCP) values were 79.9% and 84.6%, respectively. Ubiquinone 9 (Q-9) was the only respiratory quinone detected in both isolates. The major cellular fatty acids (> 10.0%) were summed feature 3 (comprising C 16:1 ω7c and/or C 16:1 ω6c), C 16:0 and C 18:1 ω9c. The polar lipid profiles of strains F26243 T and F60267 T contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidyldimethylethanolamine, phosphatidylglycerol, aminophospholipid and one unidentified phospholipid. Based on genomic characteristics, phenotypic and chemotaxonomic, strains F26243 T and F60267 T represent two novel species of the genus Marinobacter, for which the names Marinobacter sediminicola sp. nov. and Marinobacter xiaoshiensis sp. nov. are proposed, the type strains are F26243 T (= KCTC 92640 T  = MCCC 1H01345 T ) and F60267 T (= KCTC 92638 T  = MCCC 1H01346 T ).