Tissue transglutaminase_variant 2-transduced mesenchymal stem cells and their chondrogenic potential.
Ayşe Ceren Çalıkoğlu KoyuncuAyse Hande NaymanDilek TelciGamze Torun KosePublished in: Biotechnology and bioengineering (2020)
As cartilage is incapable of self-healing upon severe degeneration because of the lack of blood vessels, cartilage tissue engineering is gaining importance in the treatment of cartilage defects. This study was designed to improve cartilage tissue regeneration by expressing tissue transglutaminase variant 2 (TGM2_v2) in mesenchymal stem cells (MSC) derived from bone marrow of rats. For this purpose, rat MSCs transduced with TGM2_v2 were grown and differentiated on three-dimensional polybutylene succinate (PBSu) and poly-l-lactide (PLLA) blend scaffolds. The transduced cells could not only successfully express the short form transglutaminase-2, but also deposited the protein onto the scaffolds. In addition, they could spontaneously produce cartilage-specific proteins without any chondrogenic induction, suggesting that TGM2_v2 expression provided the cells the ability of chondrogenic differentiation. PBSu:PLLA scaffolds loaded with TGM2_v2 expressing MSCs could be used in repair of articular cartilage defects.
Keyphrases
- mesenchymal stem cells
- tissue engineering
- umbilical cord
- bone marrow
- induced apoptosis
- extracellular matrix
- cell cycle arrest
- cell therapy
- celiac disease
- stem cells
- oxidative stress
- poor prognosis
- binding protein
- endoplasmic reticulum stress
- cell death
- cell proliferation
- long non coding rna
- combination therapy
- cancer therapy
- replacement therapy