Anti-Melanogenesis Activity of Crocodile ( Crocodylus siamensis ) White Blood Cell Extract on Ultraviolet B-Irradiated Melanocytes.
Anupong JoompangPreeyanan AnwisedKunlathida LuangpraditkunNisachon JangprommaJarupa ViyochCéline ViennetSompong KlaynongsruangPublished in: Journal of medicinal food (2022)
Ultraviolet (UV) radiation generates a range of biological effects in the skin, which includes premature skin aging, hyperpigmentation, and cancer. Therefore, the development of new effective agents for UV-related skin damage remains a challenge in the pharmaceutical industry. This study aims to test the inhibitory effect of crocodile white blood cell (cWBC) extract, a rich source of bioactive peptides, on ultraviolet B (UVB)-induced melanocyte pigmentation. The results showed that cWBC (6.25-400 μ g/mL) could inhibit tyrosinase without adduct formation by 12.97 ± 4.20% on average. cWBC pretreatment (25-100 μ g/mL) had no cytotoxicity and reduced intracellular melanin to 111.17 ± 5.20% compared with 124.87 ± 7.43 for UVB condition. The protective role of cWBC pretreatment against UVB was exhibited by the promotion of cell proliferation and the prevention of UVB-induced morphological change as observed from F actin staining. The decrease of microphthalmia-associated transcription factor expression levels after cWBC pretreatment might be a mechanism by which cWBC suppresses UVB-induced pigmentation. These results suggest that cWBC could be beneficial for the prevention of UVB-induced skin pigmentation.
Keyphrases
- high glucose
- diabetic rats
- cell proliferation
- transcription factor
- oxidative stress
- drug induced
- soft tissue
- stem cells
- cell therapy
- endothelial cells
- signaling pathway
- poor prognosis
- bone marrow
- radiation therapy
- anti inflammatory
- reactive oxygen species
- long non coding rna
- radiation induced
- binding protein
- squamous cell
- dna binding
- cell migration