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Ultralong transients enhance sensitivity and resolution in Orbitrap-based single-ion mass spectrometry.

Evolène DeslignièreVictor C YinEduard H T M EbberinkAmber D RollandArjan BarendregtTobias P WörnerKonstantin O NagornovAnton N KozhinovKyle L FortYury O TsybinAlexander A MakarovAlbert J R Heck
Published in: Nature methods (2024)
Orbitrap-based charge detection mass spectrometry utilizes single-molecule sensitivity to enable mass analysis of even highly heterogeneous, high-mass macromolecular assemblies. For contemporary Orbitrap instruments, the accessible ion detection (recording) times are maximally ~1-2 s. Here by modifying a data acquisition method on an Orbitrap ultrahigh mass range mass spectrometer, we trapped and monitored individual (single) ions for up to 25 s, resulting in a corresponding and huge improvement in signal-to-noise ratio (×5 compared with 1 s), mass resolution (×25) and accuracy in charge and mass determination of Orbitrap-based charge detection mass spectrometry.
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