Selective Grafting of Protease-Resistant Adhesive Peptides on Titanium Surfaces.
Annj ZamunerAntonella PasquatoIgnazio CastagliuoloMonica DettinPaola BrunPublished in: Molecules (Basel, Switzerland) (2022)
In orthopedic, dental, and maxillofacial fields, joint prostheses, plates, and screws are widely used in the treatment of problems related to bone tissue. However, the use of these prosthetic systems is not free from complications: the fibrotic encapsulation of endosseous implants often prevents optimal integration of the prostheses with the surrounding bone. To overcome these issues, biomimetic titanium implants have been developed where synthetic peptides have been selectively grafted on titanium surfaces via Schiff base formation. We used the retro-inverted sequence (DHVPX) from [351-359] human Vitronectin and its dimer (D2HVP). Both protease-resistant peptides showed increased human osteoblast adhesion and proliferation, an augmented number of focal adhesions, and cellular spreading with respect to the control. D2HVP-grafted samples significantly enhance Secreted Phosphoprotein 1, Integrin Binding Sialoprotein, and Vitronectin gene expression vs. control. An estimation of peptide surface density was determined by Two-photon microscopy analysis on a silanized glass model surface labeled with a fluorescent analog.
Keyphrases
- gene expression
- endothelial cells
- soft tissue
- bone mineral density
- amino acid
- biofilm formation
- induced pluripotent stem cells
- mental health
- bone regeneration
- dna methylation
- pluripotent stem cells
- living cells
- systemic sclerosis
- staphylococcus aureus
- risk factors
- optical coherence tomography
- high speed
- idiopathic pulmonary fibrosis
- body composition
- pseudomonas aeruginosa
- transcription factor