Circulating B10 regulatory cells are decreased in severe and critical COVID-19.
Rodrigo Cervantes-DíazVíctor A Sosa-HernándezSandra Romero-RamírezJiram Torres-RuizAlfredo Pérez-FragosoDavid E Meza-SánchezDiana Gómez-MartínJosé Luis Maravillas-MonteroPublished in: Journal of leukocyte biology (2022)
The contribution of B cells in COVID-19 pathogenesis, beyond the production of specific antibodies against SARS-CoV-2, is still not well understood. Since one of their most relevant functional roles includes their immune-suppressive mechanisms, we decided to evaluate one of the most recognized human B regulatory subpopulations: the IL-10 + B10 cells, during COVID-19 onset. After stimulation of PBMCs for IL-10 induction, we employed multiparametric flow cytometry to determine B10 frequencies in severe and critical COVID-19 patients and then correlated those with clinical and laboratory parameters. Compared with healthy individuals, we detected a significant reduction in the B10 subset in both patient groups, which correlates with some inflammatory parameters that define the disease severity. This evidence suggests an aberrant role of B10 cells in immune responses against SARS-CoV-2 that needs to be further explained.
Keyphrases
- sars cov
- flow cytometry
- immune response
- respiratory syndrome coronavirus
- induced apoptosis
- transcription factor
- endothelial cells
- early onset
- oxidative stress
- cell cycle arrest
- case report
- coronavirus disease
- induced pluripotent stem cells
- drug induced
- dendritic cells
- endoplasmic reticulum stress
- pluripotent stem cells
- cell proliferation
- inflammatory response
- pi k akt