Brain-Derived Neurotrophic Factor Is Required for the Neuroprotective Effect of Mifepristone on Immature Purkinje Cells in Cerebellar Slice Culture.
Jennifer RakotomamonjyAbdel Mouman GhoumariPublished in: International journal of molecular sciences (2019)
Endogenous γ-aminobutyric acid (GABA)-dependent activity induces death of developing Purkinje neurons in mouse organotypic cerebellar cultures and the synthetic steroid mifepristone blocks this effect. Here, using brain-derived neurotrophic factor (BDNF) heterozygous mice, we show that BDNF plays no role in immature Purkinje cell death. However, interestingly, BDNF haploinsufficiency impairs neuronal survival induced by mifepristone and GABAA-receptors antagonist (bicuculline) treatments, indicating that the underlying neuroprotective mechanism requires the neurotrophin full expression.
Keyphrases
- cell death
- cell cycle arrest
- cerebral ischemia
- stress induced
- induced apoptosis
- poor prognosis
- spinal cord
- early onset
- subarachnoid hemorrhage
- high fat diet induced
- type diabetes
- magnetic resonance
- free survival
- skeletal muscle
- binding protein
- oxidative stress
- endoplasmic reticulum stress
- spinal cord injury
- magnetic resonance imaging
- long non coding rna
- image quality
- computed tomography