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Assays for quantification of male and female gametocytes in human blood by qRT-PCR in the absence of pure sex-specific gametocyte standards.

Claire Y T WangEmma BallardStacey LlewellynLouise MarquartTeun BousemaJames S McCarthyKatharine A Collins
Published in: Malaria journal (2020)
This study reports the validation of qRT-PCR assays that are able to accurately quantify female and male P. falciparum gametocytes at sub-microscopic densities. The assays showed excellent reproducibility, sensitivity, precision, specificity, and accuracy. The methodology will enable the estimation of gametocyte density in the absence of pure female and male gametocyte standards, and will facilitate clinical trials and epidemiological studies.
Keyphrases
  • plasmodium falciparum
  • high throughput
  • clinical trial
  • endothelial cells
  • emergency department
  • real time pcr
  • pluripotent stem cells