Jaceidin Flavonoid Isolated from Chiliadenus montanus Attenuates Tumor Progression in Mice via VEGF Inhibition: In Vivo and In Silico Studies.
Sameh S ElhadyEnas E EltamanyAmera E ShaabanAlaa A BagalagelYosra A MuhammadNorhan M El-SayedSeif N AyyadAmal A M AhmedMohamed S ElgawishSafwat A AhmedPublished in: Plants (Basel, Switzerland) (2020)
Phytochemical study of Chiliadenus montanus aerial parts afforded six compounds; Intermedeol (1), 5α-hydroperoxy-β-eudesmol (2), 5,7-dihydroxy-3,3',4'-trimethoxyflavone (3), 5,7,4'-trihydroxy-3,6,3'-trimethoxyflavone (jaceidin) (4), eudesm-11,13-ene-1β,4β,7α-triol (5) and 1β,4β,7β,11-tetrahydroxyeudesmane (6). These compounds were identified based on their NMR spectral data. The isolated compounds were tested for their cytotoxicity against liver cancer cell line (HepG2) and breast cancer cell line (MCF-7). Jaceidin flavonoid (4) exhibited the highest cytotoxic effect in vitro. Therefore, both of jaceidin and C. montanus extract were evaluated for their in vivo anti-tumor activity against Ehrlich's ascites carcinoma (EAC). Compared to control group, jaceidin and C. montanus extract decreased the tumor weight, improved the histological picture of tumor cells, lowered the levels of VEGF and ameliorate the oxidative stress. Molecular docking and in silico studies suggested that jaceidin was a selective inhibitor of VEGF-mediated angiogenesis with excellent membrane permeability and oral bioavailability.
Keyphrases
- molecular docking
- endothelial cells
- vascular endothelial growth factor
- oxidative stress
- molecular dynamics simulations
- case control
- magnetic resonance
- body mass index
- dna damage
- high resolution
- electronic health record
- anti inflammatory
- type diabetes
- optical coherence tomography
- ischemia reperfusion injury
- poor prognosis
- young adults
- big data
- solid state
- deep learning
- signaling pathway
- body weight
- mass spectrometry
- childhood cancer