Dissecting functional phenotypes of microglia and macrophages in the rat brain after transient cerebral ischemia.
Wenson D RajanBartosz WojtasBartlomiej GielniewskiAnna GieryngMalgorzata ZawadzkaBozena KaminskaPublished in: Glia (2018)
Ischemic brain injury causes local inflammation, which involves activation of resident microglia, leukocyte, and monocyte infiltration. Involvement of peripheral immune cells in ischemia-induced damage and repair is debatable. Using flow cytometry, gene expression profiling, and immunocytochemistry, we show that microglia predominate in the ischemic brain and express inflammation mediators at Day 1 after transient middle cerebral artery occlusion (MCAo) in rats. At Day 3, both resident microglia and bone marrow (BM)-derived macrophages are detected in the ischemic hemispheres and display unique transcriptomic profiles. Functional groups enriched in BM-macrophages are indicative of the pro-regenerative, immunosuppressive phenotype. Transient depletion of peripheral macrophages with clodronate-filled liposomes reduced the number of Arg1+ Iba1+ expressing cells in the ischemic brain. The analysis of microglia and macrophage signature genes shows that each cell type maintains the expression of their identity genes, even if gene expression is modified in a response to environmental clues. At Day 7, infiltrating BM-macrophages exhibit the reduced expression of Arg1, the elevated expression of iNos and many inflammatory genes, as shown by RNA sequencing. This is consistent with their switch toward a pro-inflammatory phenotype. We propose that BM-macrophages recruited to the injured brain early after ischemia could contribute to functional recovery after stroke, but they switch toward a pro-inflammatory phenotype in the ischemic parenchyma. Our results point to the detrimental role of microglia in an ischemic brain and the primarily pro-regenerative role of infiltrating BM-macrophages.
Keyphrases
- cerebral ischemia
- brain injury
- subarachnoid hemorrhage
- blood brain barrier
- inflammatory response
- genome wide
- gene expression
- oxidative stress
- neuropathic pain
- poor prognosis
- middle cerebral artery
- stem cells
- bone marrow
- mesenchymal stem cells
- genome wide identification
- flow cytometry
- single cell
- patient safety
- dna methylation
- induced apoptosis
- drug delivery
- resting state
- cell therapy
- endothelial cells
- white matter
- transcription factor
- nitric oxide
- spinal cord injury
- functional connectivity
- bioinformatics analysis
- immune response
- cell cycle arrest
- dendritic cells
- stress induced