Development of an antibiotic marker-free platform for heterologous protein production in Streptomyces.
Laura SevillanoMargarita DíazRamón I SantamaríaPublished in: Microbial cell factories (2017)
The total absence of antibiotic resistance genes makes this system a powerful tool for using Streptomyces as a host to produce proteins at the industrial level. This work is the first Streptomyces antibiotic marker-free system to be described. Graphical abstract Antibiotic marker-free platform for protein expression in Streptomyces. The antitoxin gene present in the expression plasmid counteracts the effect of the toxin gene in the genome. In absence of the expression plasmid, the toxin causes cell death ensuring that only plasmid-containing cells persist.
Keyphrases
- escherichia coli
- antibiotic resistance genes
- cell death
- poor prognosis
- wastewater treatment
- genome wide
- cell cycle arrest
- crispr cas
- induced apoptosis
- binding protein
- copy number
- high throughput
- microbial community
- genome wide identification
- long non coding rna
- risk assessment
- amino acid
- anaerobic digestion
- signaling pathway
- transcription factor
- protein protein
- single cell