Simultaneous detection of multiple mRNAs and proteins in bovine IVD cells and tissue with single cell resolution.

Kangning LiLara VardenAlthea HendersonThomas LufkinPetra Kraus

Published in: Biotechnology letters (2020)

A simple, sequential protocol combining RISH and IHC is presented that allows for simultaneous information on RNA transcripts and proteins to characterize cells within a heterogeneous cell population and complex signalling environments such as those of the IVD.

Keyphrases

single cell
induced apoptosis
cell cycle arrest
randomized controlled trial
rna seq
endoplasmic reticulum stress
signaling pathway
cell proliferation
cell death
health information
mesenchymal stem cells
pi k akt