Epithelial V-like antigen 1 promotes hepatocellular carcinoma growth and metastasis via the ERBB-PI3K-AKT pathway.
Qian-Zhi NiZhenhua ChenQianwen ZhengDong XieJing-Jing LiShu-Qun ChengXingyuan MaPublished in: Cancer science (2020)
The role of epithelial V-like antigen 1 (EVA1) has been well studied in thymic development and homostasis; however, its putative relationship with cancer remains largely unknown. Therefore, here we investigated the role of EVA1 in hepatocellular carcinoma. Interestingly, EVA1 expression was significantly increased in hepatocellular carcinoma (HCC) and was also associated with a poor prognosis and recurrence in HCC patients. Overexpression of EVA1 promoted cell growth, invasion and migration in vitro. Consistently, knockdown of EVA1 expression inhibited proliferation and migration in vitro, while repressing metastasis of HCC cells in vivo. RNA-seq analysis indicated that EVA1 is able to upregulate the expression of genes in the ERBB3-PI3K pathway. Accordingly, an increased level of AKT phosphorylation was detected in HCC cells after EVA1 overexpression. LY294002, a PI3K inhibitor, inhibited AKT phosphorylation and rescued the tumor-promoting effect of EVA1 overexpression. Altogether, the present study has revealed the oncogenic role of EVA1 during HCC progression and metastasis through the ERBB-PI3K-AKT signaling pathway, reiterating the potential use of EVA1 as a therapeutic target and/or prognostic marker for HCC.
Keyphrases
- poor prognosis
- signaling pathway
- pi k akt
- cell proliferation
- induced apoptosis
- cell cycle arrest
- long non coding rna
- rna seq
- single cell
- transcription factor
- end stage renal disease
- tyrosine kinase
- chronic kidney disease
- epithelial mesenchymal transition
- cell death
- newly diagnosed
- squamous cell carcinoma
- oxidative stress
- risk assessment
- mass spectrometry
- young adults
- papillary thyroid
- patient reported
- atomic force microscopy