Antibacterial and anti-biofilm effects of fatty acids extract of dried Lucilia sericata larvae against Staphylococcus aureus and Streptococcus pneumoniae in vitro.
Jing LiuJun JiangJunwei ZongBin LiTaowen PanYunpeng DiaoZhen ZhangXueyang ZhangMing LuShouyu WangPublished in: Natural product research (2019)
Development of new effective antimicrobial drugs is still a big challenge to date due to microbial infection remains an inevitable problem against human health. In this study, fatty acids extract of Lucilia sericata larvae (LFAs) was obtained and evaluated by gas chromatograph-mass spectrometry (GC-MS), and its antibacterial activity against Staphylococcus aureus (S. aureus) and Streptococcus pneumoniae (S. pneumoniae) was investigated. We found that LFAs exhibited effective antibacterial activity against S. aureus and S. pneumoniae with minimal inhibitory concentrations (MICs) of 125 μg/mL and 100 μg/mL, respectively. The bacterial wall and membrane were the main targets, which was confirmed by fluorescence microscopy, scanning electron microscopy and transmission electron microscopy. Furthermore, a notable anti-biofilm activity against S. aureus and S. pneumoniae was also observed, which was able to both prevent biofilm formation and eradicate mature biofilms of these bacteria. As a promising antibacterial agent, LFAs showed good application prospects in clinical practice.
Keyphrases
- electron microscopy
- staphylococcus aureus
- biofilm formation
- fatty acid
- human health
- silver nanoparticles
- candida albicans
- risk assessment
- mass spectrometry
- anti inflammatory
- clinical practice
- pseudomonas aeruginosa
- single molecule
- methicillin resistant staphylococcus aureus
- oxidative stress
- respiratory tract
- climate change
- aedes aegypti
- microbial community
- drosophila melanogaster
- liquid chromatography
- big data
- high speed
- essential oil
- high throughput
- current status
- room temperature
- atomic force microscopy
- artificial intelligence
- drug induced