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CRISPR-mediated genome editing in non-conventional yeasts for biotechnological applications.

Peng CaiJiaoqi GaoYongjin J Zhou
Published in: Microbial cell factories (2019)
Non-conventional yeasts are playing important roles as cell factories for bioproduction of biofuels, food additives and proteins with outstanding natural characteristics. However, the precise genome editing is challenging in non-conventional yeasts due to lack of efficient genetic tools. In the past few years, CRISPR-based genome editing worked as a revolutionary tool for genetic engineering and showed great advantages in cellular metabolic engineering. Here, we review the current advances and barriers of CRISPR-Cas9 for genome editing in non-conventional yeasts and propose the possible solutions in enhancing its efficiency for precise genetic engineering.
Keyphrases
  • genome editing
  • crispr cas
  • saccharomyces cerevisiae
  • genome wide
  • copy number
  • single cell
  • stem cells
  • dna methylation
  • cell therapy
  • ionic liquid
  • mesenchymal stem cells