Cryobanking European Mink ( Mustela lutreola ) Mesenchymal Stem Cells and Oocytes.
Alexandra CalleMiguel Ángel RamírezPublished in: International journal of molecular sciences (2022)
The European mink ( Mustela lutreola ) is one of Europe's most endangered species, and it is on the brink of extinction in the Iberian Peninsula. The species' precarious situation requires the application of new ex situ conservation methodologies that complement the existing ex situ and in situ conservation measures. Here, we report for the first time the establishment of a biobank for European mink mesenchymal stem cells (emMSC) and oocytes from specimens found dead in the Iberian Peninsula, either free or in captivity. New emMSC lines were isolated from different tissues: bone marrow (emBM-MSC), oral mucosa (emOM-MSc), dermal skin (emDS-MSC), oviduct (emO-MSc), endometrium (emE-MSC), testicular (emT-MSC), and adipose tissue from two different adipose depots: subcutaneous (emSCA-MSC) and ovarian (emOA-MSC). All eight emMSC lines showed plastic adhesion, a detectable expression of characteristic markers of MSCs, and, when cultured under osteogenic and adipogenic conditions, differentiation capacity to these lineages. Additionally, we were able to keep 227 Cumulus-oocyte complexes (COCs) in the biobank, 97 of which are grade I or II. The European mink MSC and oocyte biobank will allow for the conservation of the species' genetic variability, the application of assisted reproduction techniques, and the development of in vitro models for studying the molecular mechanisms of infectious diseases that threaten the species' precarious situation.
Keyphrases
- mesenchymal stem cells
- bone marrow
- adipose tissue
- umbilical cord
- infectious diseases
- gene expression
- poor prognosis
- type diabetes
- metabolic syndrome
- genome wide
- escherichia coli
- epithelial mesenchymal transition
- cystic fibrosis
- stem cells
- high fat diet
- long non coding rna
- endothelial cells
- skeletal muscle
- cell therapy
- soft tissue
- staphylococcus aureus
- cell migration