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Vidjil add-on for MRD quantification of samples processed using the EuroClonality-NGS protocol.

Guilherme Navarro Nilo GiustiAntonio Vítor RibeiroPatrícia Yoshioka JottaFlorian ThonierJosé Andres YunesJoão Meidanis
Published in: EJHaem (2023)
Assessment of minimal residual disease in acute lymphoblastic leukemia by immune repertoire NGS requires spiking CDR3 sequences at known quantities into the patient's sample. Recently, the EuroClonality-NGS group released one of the most comprehensive protocols for this purpose. ARResT/Interrogate is a closed-source software for processing these NGS libraries, developed by this same group. Vidjil, an open-source alternative, currently cannot handle libraries prepared using this protocol. Here, we present a Vidjil add-on to solve this issue. EuroClonality-NGS prepared samples analyzed with Vidjil and ARResT/Interrogate were highly concordant ( r  = 0.998) and presented low error (root-mean-square error, RMSE = 0.112).
Keyphrases
  • acute lymphoblastic leukemia
  • randomized controlled trial
  • case report
  • allogeneic hematopoietic stem cell transplantation
  • cell proliferation
  • acute myeloid leukemia