The peptidoglycan and biofilm matrix of Staphylococcus epidermidis undergo structural changes when exposed to human platelets.
Maria Loza-CorreaJuan A AyalaIris PerelmanKeith HubbardMiloslav KalabQi-Long YiMariam TahaMiguel A de PedroSandra Ramirez-ArcosPublished in: PloS one (2019)
Staphylococcus epidermidis is a bacterium frequently isolated from contaminated platelet concentrates (PCs), a blood product used to treat bleeding disorders in transfusion patients. PCs offer an accidental niche for colonization of S. epidermidis by forming biofilms and thus avoiding clearance by immune factors present in this milieu. Using biochemical and microscopy techniques, we investigated the structural changes of the peptidoglycan (PG) and the biofilm matrix of S. epidermidis biofilms formed in whole-blood derived PCs compared to biofilms grown in glucose-supplemented trypticase soy broth (TSBg). Both, the PG and the biofilm matrix are primary mechanisms of defense against environmental stress. Here we show that in PCs, the S. epidermidis biofilm matrix is mainly of a proteinaceous nature with extracellular DNA, in contrast to the predominant polysaccharide nature of the biofilm matrix formed in TSBg cultures. PG profile studies demonstrated that the PG of biofilm cells remodels during PC storage displaying fewer muropeptides variants than those observed in TSBg. The PG muropeptides contain two chemical modifications (amidation and O-acetylation) previously associated with resistance to antimicrobial agents by other staphylococci. Our study highlights two key structural features of S. epidermidis that are remodeled when exposed to human platelets and could be used as targets to reduce septic transfusions events.
Keyphrases
- biofilm formation
- candida albicans
- staphylococcus aureus
- pseudomonas aeruginosa
- escherichia coli
- endothelial cells
- single molecule
- newly diagnosed
- cystic fibrosis
- magnetic resonance
- acute kidney injury
- heavy metals
- ejection fraction
- cardiac surgery
- high resolution
- end stage renal disease
- adipose tissue
- metabolic syndrome
- magnetic resonance imaging
- pluripotent stem cells
- blood pressure
- cell cycle arrest
- oxidative stress
- computed tomography
- prognostic factors
- blood glucose
- copy number
- skeletal muscle
- risk assessment
- cell death
- antimicrobial resistance
- drinking water
- stress induced
- human health
- insulin resistance
- genome wide
- sickle cell disease
- pi k akt
- red blood cell
- atomic force microscopy
- case control