High-Precision Photothermal Ablation Using Biocompatible Palladium Nanoparticles and Laser Scanning Microscopy.
Belén Rubio-RuizAna M Pérez-LópezThomas L BrayMartin LeeAlan SerrelsMartín PrietoManuel ArrueboNeil O CarragherVictor Sebastian CabezaAsier Unciti-BrocetaPublished in: ACS applied materials & interfaces (2018)
Herein, we report a straightforward method for the scalable preparation of Pd nanoparticles (Pd-NPs) with reduced inherent cytotoxicity and high photothermal conversion capacity. These Pd-NPs are rapidly taken up by cells and able to kill labeled cancer cells upon short exposure to near-infrared (NIR) light. Following cell treatment with Pd-NPs, ablated areas were patterned with high precision by laser scanning microscopy, allowing one to perform cell migration assays with unprecedented accuracy. Using coherent Raman microscopy, cells containing Pd-NPs were simultaneously ablated and imaged. This novel methodology was combined with intravital imaging to mediate microablation of cancerous tissue in tumor xenografts in mice.
Keyphrases
- high resolution
- high speed
- induced apoptosis
- high throughput
- cell migration
- single molecule
- photodynamic therapy
- drug release
- cell cycle arrest
- drug delivery
- label free
- oxide nanoparticles
- optical coherence tomography
- cancer therapy
- single cell
- oxidative stress
- fluorescence imaging
- skeletal muscle
- metabolic syndrome
- cell proliferation
- cell therapy
- computed tomography
- fluorescent probe
- radiofrequency ablation
- liquid chromatography
- simultaneous determination
- solid phase extraction