Engineering of Radioiodine-Labeled Gold Core-Shell Nanoparticles As Efficient Nuclear Medicine Imaging Agents for Trafficking of Dendritic Cells.
Sang Bong LeeSang-Woo LeeShin Young JeongGhilSuk YoonSung Jin ChoSang Kyoon KimIn-Kyu LeeByeong-Cheol AhnJaetae LeeYong Hyun JeonPublished in: ACS applied materials & interfaces (2017)
The development of highly sensitive, stable, and biocompatible imaging agents allowing visualization of dendritic cell (DC) migration is one of the essential factors for effective DC-based immunotherapy. Here, we used a novel and efficient synthesis approach to develop radioiodine-124-labeled tannic acid gold core-shell nanoparticles (124I-TA-Au@AuNPs) for DC labeling and in vivo tracking of their migration using positron emission tomography (PET). 124I-TA-Au@AuNPs were produced within 40 min in high yield via straightforward tannic acid-mediated radiolabeling chemistry and incorporation of Au shell, which resulted in high radio-sensitivity and excellent chemical stability of nanoparticles in DCs and living mice. 124I-TA-Au@AuNPs demonstrated good DC labeling efficiency and did not affect cell biological functions, including proliferation and phenotype marker expression. Importantly, 124I-TA-Au@AuNPs in an extremely low amount (0.1 mg/kg) were successfully applied to track the migration of DCs to lymphoid organs (draining lymph nodes) in mice.
Keyphrases
- dendritic cells
- positron emission tomography
- sensitive detection
- pet imaging
- computed tomography
- reduced graphene oxide
- immune response
- regulatory t cells
- high resolution
- lymph node
- pet ct
- poor prognosis
- high fat diet induced
- single cell
- signaling pathway
- gold nanoparticles
- cell therapy
- quantum dots
- ionic liquid
- insulin resistance
- drug delivery
- visible light
- fluorescence imaging
- binding protein
- fluorescent probe
- bone marrow
- rectal cancer
- early stage
- living cells
- metabolic syndrome
- drug discovery
- simultaneous determination