Chemical synthesis of per-selenocysteine human epidermal growth factor.
Toshiki TakeiHideaki TanakaNobuaki OkumuraToshifumi TakaoLuis MoroderHironobu HojoPublished in: Journal of peptide science : an official publication of the European Peptide Society (2022)
Human seleno-epidermal growth factor (seleno-EGF), a 53-residue peptide where all six cysteine residues of the parent human EGF sequence were replaced by selenocysteines, was synthesized and the oxidative folding of a polypeptide containing three diselenide bonds was compared to that of the parent cysteine peptide. The crude HPLC profiles clearly showed that both the native EGF and its selenocysteine-analogue fold smoothly, yielding a single sharp peak, proving that even in the case of three disulfide-bonded polypeptides the disulfide-to-diselenide bond substitution is highly isomorphous, as confirmed by conformational circular dichroism measurements and particularly by the biological assays.