Rapid on-site dual optical system to measure specific reactive oxygen species (O2-• and OCl-) in a tiny droplet of whole blood.
Kimiko KazumuraKozo TakeuchiAkiko HaraToshiyuki MiwaMasaki HattoriYuqiu WuNaokazu MorishitaHiroshi TsuchiyaToshihiko OsawaPublished in: PloS one (2018)
Oxidative stress has been implicated in various disorders and controlling it would be important for healthy life. We have developed a new optical system for easily and accurately measuring oxidative stress in whole blood. It is optimized for simultaneously detecting reactive oxygen species (ROS) and highly reactive ROS (hROS), elicited mostly by white blood cells in a few microliters of blood. Results obtained by using this system show at least four important findings. 1) chemiluminescence of MCLA was confirmed to be attributable to O2-•. 2) PMA-stimulated cells released O2-• longer and more slowly than fMLP-stimulated ones. 3) fluorescence produced by APF oxidation was confirmed to be attributable to hROS, mostly OCl-, produced by myeloperoxidase. 4) the generation of OCl- was found to be a slower process than the O2-• generation. We also conducted pilot studies of oxidative stress in healthy volunteers.
Keyphrases
- reactive oxygen species
- induced apoptosis
- oxidative stress
- dna damage
- endoplasmic reticulum stress
- cell cycle arrest
- cell death
- ischemia reperfusion injury
- diabetic rats
- high resolution
- high speed
- randomized controlled trial
- cell proliferation
- clinical trial
- single molecule
- single cell
- study protocol
- energy transfer
- sensitive detection
- case control
- heat shock
- quantum dots